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Postdoctoral Fellow | Structural Biology Job @ AstraZeneca, Alderley Park, UK: PhD and/or MD in structural biology, preferably biomolecular NMR...

Postdoctoral Fellow | Structural Biology Job @ AstraZeneca, Alderley Park

Postdoctoral Fellow | Structural Biology Job @ AstraZeneca, Alderley Park, UK: At AstraZeneca, we believe in the potential of people who share our passion for science. By thinking boldly and working collaboratively to pursue discoveries beyond imagination, you’ll develop further and faster than you thought possible. We’ll complement your drive and determination to meet unmet patient needs around the world with the inspiration and opportunities to enhance your niche expertise and build your skills. So if you’re curious about what science can do, come and make a difference.

Our Discovery Sciences team operates from truly state-of-the-art centers spanning the UK, US, and Sweden. You’ll work with some of the most knowledgeable technology experts in the industry, all collaborating on high-profile drug discovery projects. With a wide range of responsibilities, from sample management and screening to bio-reagent supply, the opportunities to develop your expertise here are limitless.

Postdoctoral Fellow | Structural Biology Job @ AstraZeneca, Alderley Park

Main duties and responsibilities

The Poly-ADP ribose polymerase (PARP) family of proteins are well known enzymes in the DNA damage repair pathway (DDR); PARP proteins also play an important role in maintaining chromatin architecture and facilitating gene transcription in the absence of DNA damage. PARPs directly interact with DNA breaks but it is not known how they are targeted to sites of de novo transcriptional activation. You’ll be part of a project that aims to investigate the structure and function of the PARP ADP-ribosylases with respect to their interactions with the nucleosome.

You’ll initially focus on producing recombinant PARP proteins and nucleosomes, the latter bearing histone modification mimics. You’ll map the interaction interface between PARP and nucleosomes via biomolecular NMR and in parallel the structure of the complex will be pursued by cryo-electron microscopy.

Carrying out biophysical and enzymatic characterisation of the nucleosome:PARP complexes will be part of the role. In order to demonstrate the relationship between PARP proteins and relevant nucleosome modifications in cells, it might be possible to extend the project to include RT-PCR or ChIP-Seq, depending on your expertise.

Structural studies will involve collaborating with the group of Dr Steve Gamblin based at The Francis Crick Institute. The Gamblin group has a long history of working on chromatin remodelling complexes and offer both invaluable scientific input and access to state of the art facilities at their London laboratories.

You’ll initially be based at Alderley Park in Cheshire until completion of our new facilities in Cambridge. Alderley Park houses the AZ UK biomolecular NMR group (two 600 and one 800 cryoprobe equipped spectrometers) and the RAD protein reagents group. These groups will give you access to considerable expertise and equipment, while your research will give you the opportunity to influence fellow scientists across many disciplines.

Essential requirements

  • PhD and/or MD in structural biology, preferably biomolecular NMR, or extensive post-graduate experience in this area
  • Skills in recombinant protein expression, purification and characterisation
  • Practical experience of biomolecular NMR
  • Experience with biophysical techniques (SPR, ITC etc.) and enzyme kinetics (assay design and implementation)
  • Interest in furthering the understanding of chromatin regulation
  • Strong interpersonal and organisational skills
  • Proven ability to independently design and execute informative experiments and interpret results
  • Strong commitment to science, with ability to work both independently and within a team environment.

Desirable requirements

  • Experience of recombinant nucleosome production or protein refolding
  • Experience of mammalian cell culture and RT-PCR/ChIP-Seq.
  • Experience of Cryo-EM techniques
  • A strong scientific track record, evidenced by published or accepted papers in peer-reviewed journals.

To apply for this position, please click the apply link below.

AstraZeneca is an equal opportunity employer. AstraZeneca will consider all qualified applicants for employment without discrimination on grounds of disability, sex or sexual orientation, pregnancy or maternity leave status, race or national or ethnic origin, age, religion or belief, gender identity or re-assignment, marital or civil partnership status, protected veteran status (if applicable) or any other characteristic protected by law. AstraZeneca only employs individuals with the right to work in the country/ies where the role is advertised.

This is a 2-year Fixed Term Contract opportunity, plus 1 year which will be merit based.  The role will be based at Alderley Park with a competitive salary on offer

Opening date of advert:  17th February 2017

Closing date of advert:   18th April 2017

Postdoctoral Fellow | Structural Biology Job @ AstraZeneca, Alderley Park

If you share our passion for scientific excellence, find out more at careers.astrazeneca.com

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