Research Associate Protein Production @ Spotlight Therapeutics, Hayward, CA: We are hiring a Senior Research Associate (Bachelors or Masters in Biochemistry/Molecular Biology, or related field) to streamline our protein production efforts. This position will support protein engineering Scientists and work interactively with other scientific teams at Spotlight.

Research Associate Protein Production @ Spotlight Therapeutics, Hayward, CA

The ideal candidate will have 5-10 years experience generating a purified recombinant protein, starting from construct design, proceeding through laboratory scale purification/optimization, culminating in production through bacterial and eukaryotic (e.g. cerevisiae, Picchia, CHO, HEK-293, and hybridoma) expression systems.

Research experience generating protein for structural biology, ability to incorporate structure-function data to guide design and construct optimization, and experience optimizing the solubility and expression/yield of difficult to purify proteins is strongly desired.

In addition, experience with molecular biology techniques (Type-II cloning, GoldenGate, Gibson assembly, etc), and methods to streamline generating and evaluating a large number of protein expression constructs are highly desired.

Experience designing and building high-throughput, arrayed protein production workflows, biochemical and biophysical characterization of protein and RNA complexes will be an added plus.

Research Associate Protein Production @ Spotlight Therapeutics, Hayward, CA


  • Generating purified recombinant protein.
  • Scaling purification from test batches to multiple liter productions.
  • Optimizing constructs to improve expression and purity.
  • Assistance with protein variant screening to discover new biotherapeutics.
  • Developing high-throughput assays.
  • Automating or streamlining repetitive tasks.


  • RA candidates should have at least two years of relevant experience.
  • SRA candidates should have 5+ years of relevant experience.
  • Protein purification experience required.
  • Molecular biology (cloning) expertise required.
  • Prior experience with structure/function investigation desired.
  • Experience generating protein for crystallography desired.
  • Experience optimizing solubility and expression desired.
  • Protein variant screening experience desired.
  • Automation experience desired.


Bachelor’s or advanced degree.

Research Associate Protein Production @ Spotlight Therapeutics, Hayward, CA


To apply, send a copy of your CV to [email protected]

This hiring window will be open until August 1st, 2018.

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