Ph.D. in Biochemical Engineering, with 6+ years relevant experience and a thorough knowledge of Process Technology.
Skilled in both protein purification for R&D purposes, as well as purification scale-up process development for technology transfer to manufacturing. Highly versed and polished in the execution of downstream multi-steps purification of novel therapeutic proteins to support early phase clinical trials studies. Strong background in the development of protein purification protocols (AKTA-based protein chromatography) at small and larger scale. An AKTA/UNICORN expert across all models and software versions. Experienced with protein analytics, UF/DF, filtration, and formulation.
- Recombinant proteins, monoclonal antibodies, and nucleic acid (pDNA and mRNA) purification
- Different modes of chromatography
- Bead-, fiber-, monoliths- and membrane-based adsorbents
- Synthesis and evaluation of ion-exchangers, and affinity-based adsorbents
- Basic analytical techniques for protein and nucleic acid (HPLC analysis, protein and nucleic acid quantification methods, electrophoresis, etc.)
- Mass spectrometry characterization of therapeutic antibodies and other recombinant proteins
- Scale-up /-down
- Working knowledge of good laboratory practices and cGMP
- Tangential flow filtration (TFF), Ultrafiltration, Diafiltration
Keywords: Process development, bioanalytical scientist, process engineer, downstream engineer, protein purification, senior scientist, biotechnology, Downstream Processing of Biopharmaceuticals.
Feb. 2017 – Present
- Modification of porous nylon membranes/monoliths with LbL polyelectrolytes for rapidly capturing monoclonal antibodies.
- Mass spectrometry characterization of therapeutic antibodies and other recombinant proteins.
May 2016 – Oct. 2016
- Synthesis and evaluation of affinity-based adsorbents for purification of mAbs.
Jun. 2011 – Apr. 2015
- Synthesis and evaluation of ion-exchangers, and affinity-based chromatography adsorbents.
- Protein and nucleic acid purification – monoliths, membrane and resin-based adsorbents.
Jul. 2010 – Jan. 2011
- Experienced working with purification of recombinant protein [Insulin Analogue (Glargine)]
- Experienced working within an aseptic environment
- Experienced working with and following SOPs and batch records
- Experienced working within a cGMP and safety first environment
- Trained on all column operations (Ion-, buffer-, cation-exchange chromatography, and RP-HPLC
- Conversion Reaction and Bulk Isolation
- Trained on testing and assembling of both vent and liquid filters
- Perform Aseptic sampling of eluates and buffers
- Operation of Analytical HPLC
- Excellent process and problem-solving skills
- Strong ability to work in teams
Thesis: “Preparation and characterization of grafted adsorbents and their applications in biomolecule purification”.
Supervisor: Prof. Dr. Marcelo Fernández-Lahore.
Within this project, Hybrid Disposable Cartridges (HDC), a hybrid approach created by merging existing textile technology with Material/polymer chemistry was used to generate a cost-effective and disposable system supporting eco-friendly bioprocessing.
The goal here was to evaluate the performance of gPore NW materials, which can be functionalized and used to purify a range of bioproducts efficiently.
The further objective was to tailor the gPore NW system, which has already shown excellent results in the recovery and purification of natural as well as recombinant proteins, to other products, like pDNA or monoclonal antibodies.
Thesis: “Regulation of T cell immune responses by chemokines CCL19 and CCL21”.
Supervisor: Dr. Bhaskar Saha, National Centre for Cell Science, Pune.
July 2009 – Sept. 2009
3 month foundation certified program in Genomics.
2012 5th Monolith Summer School & Symposium – MSS2012, Slovenia.
2007 Modern Approaches and Innovations in Biotechnology and Biodiversity conservation, India.
2016 European Society for Biochemical Engineering Sciences (ESBES) 2016, Ireland.
2014 16th European Congress on Biotechnology, Scotland.
2016 “QbD Related Modelling and Control Strategies” at the ESBES 2016, Ireland.
2012 “Purification of Biomolecules and scale-up” at the MSS2012, Slovenia.
N. K. Singh, R. N. Dsouza, M. L. Sánchez, S. Verma, E. Achilli, R. R. Vennapusa, M. Grasselli, and M. Fernández-Lahore, “Preparation and Characterization of Grafted Cellulosic Fibers and their Applications in Protein Purification“, Sep. Purif. Technol., 143 (2015) pp. 177–183.
N. K. Singh, R. N. Dsouza, N. S. Bibi, and M. Fernández-Lahore, “Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography“, inAffinity Chromatography (Ed.: S. Reichelt), Spinger, New York, USA, Methods in Molecular Biology, 1286 (2015) pp. 201–212.
N. K. Singh, R. N. Dsouza, M. Grasselli, and M. Fernández-Lahore, “Gamma ray mediated functionalization of monolithic cryogels for macro-biomolecule purification“, N. Biotechnol., 31, Supplement (2014) pp. S127.
N. K. Singh, R. N. Dsouza, M. Grasselli, and M. Fernández-Lahore, “High capacity cryogel-type adsorbents for protein purification“, J. Chromatogr. A, 1355 (2014) pp. 143–148.
N. S. Bibi, N. K. Singh, R. N. Dsouza, M. Aasim, and M. Fernández-Lahore, “Synthesis and performance of megaporous immobilized metal-ion affinity cryogels for recombinant protein capture and purification“, J. Chromatogr. A, 1272 (2013) pp. 145–149.
- Linkedin: https://www.linkedin.com/in/navinkbiotech
- ResearchGate: https://www.researchgate.net/profile/Naveen_Kumar_Singh
- Researcher ID: http://www.researcherid.com/rid/H-9521-2015
- Google Scholar: https://scholar.google.co.in/citations?user=vamkCWsAAAAJ&hl=en&oi=sra
- Academia: Follow me on Academia.edu
- Twitter: https://twitter.com/nsmalhan/