Dr. Naveenkumar Singh
Biochemical Engineer

Ph.D. in Biochemical Engineering, with 6+ years relevant experience and a thorough knowledge of Process Technology.

Skilled in both protein purification for R&D purposes, as well as purification scale-up process development for technology transfer to manufacturing. Highly versed and polished in the execution of downstream multi-steps purification of novel therapeutic proteins to support early phase clinical trials studies. Strong background in the development of protein purification protocols (AKTA-based protein chromatography) at small and larger scale. An AKTA/UNICORN expert across all models and software versions. Experienced with protein analytics, UF/DF, filtration, and formulation.

Specialties:

  • Recombinant proteins, monoclonal antibodies, and nucleic acid (pDNA and mRNA) purification
  • Different modes of chromatography
  • Bead-, fiber-, monoliths- and membrane-based adsorbents
  • Synthesis and evaluation of ion-exchangers, and affinity-based adsorbents
  • Basic analytical techniques for protein and nucleic acid (HPLC analysis, protein and nucleic acid quantification methods, electrophoresis, etc.)
  • Mass spectrometry characterization of therapeutic antibodies and other recombinant proteins
  • Scale-up /-down
  • Working knowledge of good laboratory practices and cGMP
  • Tangential flow filtration (TFF), Ultrafiltration, Diafiltration

Keywords: Process development, bioanalytical scientist, process engineer, downstream engineer, protein purification, senior scientist, biotechnology, Downstream Processing of Biopharmaceuticals.

Experience

Feb. 2017 – Present

Postdoctoral Research Associate
University of Notre Dame
  • Modification of porous nylon membranes/monoliths with LbL polyelectrolytes for rapidly capturing monoclonal antibodies.
  • Mass spectrometry characterization of therapeutic antibodies and other recombinant proteins.

May 2016 – Oct. 2016

Research Scientist (Postdoc)
Jacobs University Bremen, Bremen, Germany
  • Synthesis and evaluation of affinity-based adsorbents for purification of mAbs.

Jun. 2011 – Apr. 2015

Research Associate
Jacobs University Bremen, Bremen, Germany
  • Synthesis and evaluation of ion-exchangers, and affinity-based chromatography adsorbents.
  • Protein and nucleic acid purification – monoliths, membrane and resin-based adsorbents.

Jul. 2010 – Jan. 2011

Trainee and Executive Trainee
Wockhardt Ltd, Aurangabad (M.S.), India
  • Experienced working with purification of recombinant protein [Insulin Analogue (Glargine)]
  • Experienced working within an aseptic environment
  • Experienced working with and following SOPs and batch records
  • Experienced working within a cGMP and safety first environment
  • Trained on all column operations (Ion-, buffer-, cation-exchange chromatography, and RP-HPLC
  • Conversion Reaction and Bulk Isolation
  • Trained on testing and assembling of both vent and liquid filters
  • Perform Aseptic sampling of eluates and buffers
  • Operation of Analytical HPLC
  • Excellent process and problem-solving skills
  • Strong ability to work in teams

Education

2011–2016

Ph.D. in Biochemical Engineering
Jacobs University, Bremen, Germany

Thesis: “Preparation and characterization of grafted adsorbents and their applications in biomolecule purification”.

Supervisor: Prof. Dr. Marcelo Fernández-Lahore.

Within this project, Hybrid Disposable Cartridges (HDC), a hybrid approach created by merging existing textile technology with Material/polymer chemistry was used to generate a cost-effective and disposable system supporting eco-friendly bioprocessing.

The goal here was to evaluate the performance of gPore NW materials, which can be functionalized and used to purify a range of bioproducts efficiently.

The further objective was to tailor the gPore NW system, which has already shown excellent results in the recovery and purification of natural as well as recombinant proteins, to other products, like pDNA or monoclonal antibodies.

2007–2009

M.Sc. in Biotechnology
Jaipur National University, Jaipur, Rajasthan, India

Thesis: “Regulation of T cell immune responses by chemokines CCL19 and CCL21”.

Supervisor: Dr. Bhaskar Saha, National Centre for Cell Science, Pune.

2004–2007

B.Sc. in Biotechnology
University of Pune, Pune, Maharashtra, India
Training

July 2009 – Sept. 2009

Trainee
ILS Bioservices, Gurgaon, Haryana, India

3 month foundation certified program in Genomics.

Conferences and Workshops
Talks at Conferences

2012 5th Monolith Summer School & Symposium – MSS2012, Slovenia.

2007 Modern Approaches and Innovations in Biotechnology and Biodiversity conservation, India.

Posters at Conferences

2016 European Society for Biochemical Engineering Sciences (ESBES) 2016, Ireland.

2014 16th European Congress on Biotechnology, Scotland.

Workshops

2016 “QbD Related Modelling and Control Strategies” at the ESBES 2016, Ireland.

2012 “Purification of Biomolecules and scale-up” at the MSS2012, Slovenia.

Publications

N. K. Singh, R. N. Dsouza, M. L. Sánchez, S. Verma, E. Achilli, R. R. Vennapusa, M. Grasselli, and M. Fernández-Lahore, “Preparation and Characterization of Grafted Cellulosic Fibers and their Applications in Protein Purification“, Sep. Purif. Technol., 143 (2015) pp. 177–183.

N. K. Singh, R. N. Dsouza, N. S. Bibi, and M. Fernández-Lahore, “Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography“, inAffinity Chromatography (Ed.: S. Reichelt), Spinger, New York, USA, Methods in Molecular Biology, 1286 (2015) pp. 201–212.

N. K. Singh, R. N. Dsouza, M. Grasselli, and M. Fernández-Lahore, “Gamma ray mediated functionalization of monolithic cryogels for macro-biomolecule purification“, N. Biotechnol., 31, Supplement (2014) pp. S127.

N. K. Singh, R. N. Dsouza, M. Grasselli, and M. Fernández-Lahore, “High capacity cryogel-type adsorbents for protein purification“, J. Chromatogr. A, 1355 (2014) pp. 143–148.

N. S. Bibi, N. K. Singh, R. N. Dsouza, M. Aasim, and M. Fernández-Lahore, “Synthesis and performance of megaporous immobilized metal-ion affinity cryogels for recombinant protein capture and purification“, J. Chromatogr. A, 1272 (2013) pp. 145–149.

Get in Touch

Technical Competencies

Downstream Processing

Preparative chromatography (monoliths-, membrane-, and bead-based adsorbents)(ion-exchange, affinity, multimodal and hydrophobic-interactions), size-exclusion, reversed-phase chromatography, tangential flow filtration (TFF), ultrafiltration, diafiltration, centrifugation, HPLC.

Analytical Techniques

SDS-PAGE, Native PAGE, Western blotting, Southern blotting, Northern blotting, isoelectric focusing, spectrophotometry, confocal microscopy, ELISA, and ExperionTMautomated electrophoresis system.

Molecular Biology

Nucleic acid isolation and processing, cloning, reverse transcriptase-PCR.

Cell Culture

Bacterial cell culture, primary cultures (Murine Macrophages, T cells, Dendritic cells, Leishmania, etc.) and cell lines.

Fermentation

Batch and Fed-Batch fermentation (upto 500 L).

Systems

Äkta (Explorer/Pilot), DanProcess/BioProcess MPLC/HPLC systems, and LTQ Orbitrap Velos Mass Spectrometry.

Experience in GLP, cGMP, standard operating procedures (SOP’s), health and safety documentation.

Operating system (Computer)

Linux, MacOS and Windows.

Publishing (Computer)

html, LATEX, Microsoft Office, EndNote, Adobe Illustrator, Adobe Photoshop.

Scientific (Computer)

Origin, ChemBioOffice, MODDE, BLAST, FASTA, PROSITE, UNICORN.

Languages (Computer)

C, C++.

Website Designing (Computer)

Blogger, WordPress, Microsoft WebMatrix.

English (Languages)
Hindi (Languages)
Marathi (Languages)
German (Languages)
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