Dr. Naveen K Singh
Biochemical Engineer

Naveen K Singh did his Ph.D. (Biochemical Engineering) from Jacobs University Bremen, Germany. He holds his passion for Biochemical Engineering and likes to connect with the professionals in different fields for constantly evaluating the future of Bio-Entrepreneurship.

Experience

Feb. 2017 – Present

Postdoctoral Research Associate
University of Notre Dame

May 2016 – Oct. 2016

Research Scientist (Postdoc)
Jacobs University Bremen, Bremen, Germany
  • Synthesis and evaluation of affinity-based adsorbents for purification of mAbs.

Jun. 2011 – Apr. 2015

Research Associate
Jacobs University Bremen, Bremen, Germany
  • Synthesis and evaluation of ion-exchangers, and affinity-based chromatography adsorbents.
  • Protein and nucleic acid purification – monoliths, membrane and resin-based adsorbents.

Jul. 2010 – Jan. 2011

Trainee and Executive Trainee
Wockhardt Ltd, Aurangabad (M.S.), India
  • Large scale production and purification of recombinant insulin analogue glargine.
  • Follow and maintain cGMP, standard operating procedures, health and safety policies.

Education

2011–2016

Ph.D. in Biochemical Engineering
Jacobs University, Bremen, Germany

Thesis: “Preparation and characterization of grafted adsorbents and their applications in biomolecule purification”.

Supervisor: Prof. Dr. Marcelo Fernández-Lahore.

2007–2009

M.Sc. in Biotechnology
Jaipur National University, Jaipur, Rajasthan, India

Thesis: “Regulation of T cell immune responses by chemokines CCL19 and CCL21”.

Supervisor: Dr. Bhaskar Saha, National Centre for Cell Science, Pune.

2004–2007

B.Sc. in Biotechnology
University of Pune, Pune, Maharashtra, India
Training

July 2009 – Sept. 2009

Trainee
ILS Bioservices, Gurgaon, Haryana, India

3 month foundation certified program in Genomics.

Conferences and Workshops
Talks at Conferences

2012 5th Monolith Summer School & Symposium – MSS2012, Slovenia.

2007 Modern Approaches and Innovations in Biotechnology and Biodiversity conservation, India.

Posters at Conferences

2016 European Society for Biochemical Engineering Sciences (ESBES) 2016, Ireland.

2014 16th European Congress on Biotechnology, Scotland.

Workshops

2016 “QbD Related Modelling and Control Strategies” at the ESBES 2016, Ireland.

2012 “Purification of biomolecules and scale-up” at the MSS2012, Slovenia.

Publications

N. K. Singh, R. N. Dsouza, M. L. Sánchez, S. Verma, E. Achilli, R. R. Vennapusa, M. Grasselli, and M. Fernández-Lahore, “Preparation and Characterization of Grafted Cellulosic Fibers and their Applications in Protein Purification“, Sep. Purif. Technol., 143 (2015) pp. 177–183.

N. K. Singh, R. N. Dsouza, N. S. Bibi, and M. Fernández-Lahore, “Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography“, inAffinity Chromatography (Ed.: S. Reichelt), Spinger, New York, USA, Methods in Molecular Biology, 1286 (2015) pp. 201–212.

N. K. Singh, R. N. Dsouza, M. Grasselli, and M. Fernández-Lahore, “Gamma ray mediated functionalization of monolithic cryogels for macro-biomolecule purification“, N. Biotechnol., 31, Supplement (2014) pp. S127.

N. K. Singh, R. N. Dsouza, M. Grasselli, and M. Fernández-Lahore, “High capacity cryogel-type adsorbents for protein purification“, J. Chromatogr. A, 1355 (2014) pp. 143–148.

N. S. Bibi, N. K. Singh, R. N. Dsouza, M. Aasim, and M. Fernández-Lahore, “Synthesis and performance of megaporous immobilized metal-ion affinity cryogels for recombinant protein capture and purification“, J. Chromatogr. A, 1272 (2013) pp. 145–149.

Get in Touch

Technical Competencies

Downstream Processing

Preparative chromatography (monoliths-, membrane-, and bead-based adsorbents)(ion-exchange, affinity, multimodal and hydrophobic-interactions), size-exclusion, reversed-phase chromatography, tangential flow filtration (TFF), ultrafiltration, diafiltration, centrifugation, HPLC.

Analytical Techniques

SDS-PAGE, Native PAGE, Western blotting, Southern blotting, Northern blotting, isoelectric focusing, spectrophotometry, confocal microscopy, ELISA, and ExperionTMautomated electrophoresis system.

Molecular Biology

Nucleic acid isolation and processing, cloning, reverse transcriptase-PCR.

Cell Culture

Bacterial cell culture, primary cultures (Murine Macrophages, T cells, Dendritic cells, Leishmania, etc.) and cell lines.

Fermentation

Batch and Fed-Batch fermentation (upto 500 L).

Systems

Äkta (Explorer/Pilot), DanProcess/BioProcess MPLC/HPLC systems, 433A Peptide Synthesizer and autoflex II (MALDI-TOF).

Experience in GLP, cGMP, standard operating procedures (SOP’s), health and safety documentation.

Operating system (Computer)

Linux, MacOS and Windows.

Publishing (Computer)

html, LATEX, Microsoft Office, EndNote, Adobe Illustrator, Adobe Photoshop.

Scientific (Computer)

Origin, ChemBioOffice, MODDE, BLAST, FASTA, PROSITE, UNICORN.

Languages (Computer)

C, C++.

Website Designing (Computer)

Blogger, WordPress, Microsoft WebMatrix.

English (Languages)
Hindi (Languages)
Marathi (Languages)
German (Languages)
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